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    回腸標準可消化組氨酸/賴氨酸對斷奶仔豬(7-11kg)生長性能、腸道健康和含組氨酸蛋白動員的影響

    發布單位:天津瑞孚農牧科技集團有限公司

    查看次數:2054

    時間:2023-02-17
          試驗目的在于評估高于NRC需要量的回腸標準可消化組氨酸/賴氨酸(SID H/L)對斷奶仔豬(7-11kg)生長性能、腸道健康和含組氨酸蛋白(包括血紅蛋白、肌肽和胰蛋白酶原)動員的影響。
          試驗選用40 頭斷奶仔豬(26日齡;初重7.1 ± 0.5 kg),根據性別和初始體重的完全隨機區組設計,分配至5種日糧處理。日糧中SID H/L分別為 26%、32%、38%、43% 和 49%,并飼喂14 天,日糧SID Lys含量為1.22%。在第0、7和 14 天分別記錄采食量和體重。第 12 天采集血樣。第14天對豬實施安樂死,采集胰腺、背最長肌、空腸中段和空腸粘膜樣品。使用SAS的Proc Mixed對數據進行統計分析。
          日糧處理對仔豬生長性能沒有顯著影響,但是SID H/L顯著影響血紅蛋白水平(P<0.05,36%時濃度最高,為12 g/dL)、空腸粘膜中免疫球蛋白A水平(IgA,P<0.05,35%時濃度最低,為1.25 μg/mg),空腸中絨毛高度(P=0.065,40%時最大,為536 μm)、胰腺中胰蛋白酶原含量(P=0.083,41%時含量最高,為242 pg/mg)和肌肉中肌肽水平(P=0.051,38%時濃度最高,為4.7 ng/mg)。日糧SID H/L線性提高肌肉中的蛋白質羰基(P<0.05,從1.95到2.80 nmol/mg),并減少腸細胞增殖(P<0.05,從29.1%到26.9%)。
          總之,回腸標準可消化組氨酸/賴氨酸在35%-41%之間可以改善仔豬(7-11kg)腸道健康,并使含組氨酸蛋白水平達到峰值,這表明含組氨酸的蛋白質是確定組氨酸需要量時的有效標準。

    The effects of standardized ileal digestible His to Lys ratio on growth performance, intestinal health, and mobilization of histidine-containing proteins in pigs at 7 to 11 kg body weight
    The objectives were to evaluate the effects of standardized ileal digestible (SID) His:Lys ratio above the current NRC requirement on growth performance, intestinal health, and mobilization of His-containing proteins, including hemoglobin, carnosine, and trypsinogen, in nursery pigs from 7 to 11 kg body weight (BW). Forty pigs (26 d of age; initial BW of 7.1?±?0.5 kg) were allotted to 5 dietary treatments based on a randomized complete block design with sex and initial BW as blocks. Dietary treatments were supplemented with varying SID His to Lys ratios of 26%, 32%, 38%, 43%, and 49% and fed to pigs for 14 d (SID Lys?=?1.22%). Feed intake and BW were recorded at d 0, 7, and 14 to measure growth performance. Blood samples were collected on d 12. Pigs were euthanized on d 14 to collect pancreas, longissimus dorsi muscles, mid-jejunum, and jejunal mucosa. Data were analyzed using the Proc Mixed of SAS. Growth performance was not affected, whereas varying SID His to Lys ratio affected hemoglobin (P?<?0.05, max: 12 g/dL at 36%), immunoglobulin A (IgA, P?<?0.05, min: 1.25 μg/mg at 35%) in jejunal mucosa, villus height (P?=?0.065, max: 536 μm at 40%) in jejunum, trypsinogen (P?=?0.083, max: 242 pg/mg at 41%) in pancreas, and carnosine (P?=?0.051, max: 4.7 ng/mg at 38%) in muscles. Varying SID His to Lys ratios linearly increased (P?<?0.05, from 1.95 to 2.80 nmol/mg) protein carbonyl in muscles and decreased (P?<?0.05, from 29.1% to 26.9%) enterocyte proliferation. In conclusion, SID His to Lys ratio between 35% and 41% in diets fed to nursery pigs at 7 to 11 kg enhanced intestinal health and maximized concentrations of His-containing proteins, indicating that His-containing proteins are effective response criteria when determining His requirement.

    文章來源:豬營養國際論壇
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